Figure 2Figure 2
Neutrophil-microglial contacts in lumbar parenchyma, AQP4-IgG infusion day 3. ( A ) Confocal image identifies putatively interacting microglia (Cx3cr1GFP + , green) and neutrophil (Ly6G + , red) adjacent to a neuronal soma (NeuN + , blue); subsequent images are Imaris 3D rendering. ( B ) Cord region of interest containing contacting neutrophil-microglia; laser-branded frame by 2-photon imaging; magnified. ( C ) Serial block-face scanning electron microscopy (SBF-SEM) shows ultrastructurally the same neutrophil, microglia, and 2 neurons boxed in A . Yellow arrows (magnified, C1 ) indicate contacting microglial-neutrophil somata edges (green and red lines in C2 ). ( D ) 3D serial reconstruction of contacting microglial-neutrophil somata in C ( Supplemental Video 1 , Z -stack). ( E ) Representative confocal image (left, 2,048 × 2,048 pixel; ×63 objective lens) and 3D rendering image (right) show microglial process and soma interacting with Ly6G + neutrophils in lumbar parenchyma of