Figure 6Figure 6
C5ar1 deficiency ameliorates neuronal dysfunction and lipid droplet accumulation in motor neurons. ( A ) Experimental design: mice were infused continuously with AQP4-IgG using osmotic pumps from day 0 through day 7. ( B – D ) Quantification of Nissl bodies ( B ), Nissl area ( C ), and percentage area occupied by ChAT + motor neurons in gray matter of lumbar cord of Ctrl-IgG–infused mice at day 3, AQP4-IgG–infused mice at days 3, 14, and 28. ( E – H ) Representative images of Nissl bodies (red) in ventral gray matter (vGM) neurons of wild-type and C5ar1 –/– mice at day 3. Boxed areas are enlarged on the right. ( I and J ) Numbers and sizes of Nissl body + neurons in vGM ( n = 8 mice per group). ( K ) Representative motor neuron confocal images and quantification in vGM of WT mice infused with Ctrl-IgG or AQP4-IgG and C5ar1 –/– mice infused with AQP4-IgG or control-IgG (not shown). HuD + (green); ChAT + (magenta). ( L and M ) Numbers of ChAT + motor neurons (MNs) and thei