Fig. 3Figure 3
A: Histogram of the most abundant proteins measured in astrocyte ApoE lipoproteins. Abundance was determined by excluding common contaminants, showing differential expression compared to proteins measured in ApoE −/− astrocyte-conditioned media, and the protein abundance being greater than the total mean + 0.5 standard deviation threshold. Error bars represent mean ± SEM. For proteomics analysis, the following groups were analyzed: ApoE2 astrocytes (n = 5), ApoE3 astrocytes (n = 5), and ApoE4 astrocytes (n = 5). B: PCA of the proteome of astrocyte ApoE lipoproteins. Circles are colored based on the ApoE isoform and represent the 95% confidence interval. Astrocyte samples largely overlap in their proteome, suggesting that the ApoE isoform is not a major determinant of the proteome of astrocyte-secreted ApoE lipoproteins. C: Volcano plot showing differential protein abundance of proteins bound to astrocyte ApoE2 immunopurified lipoproteins versus astrocyte ApoE3 immunopurified lipoprote