Fig. 5Figure 5
A: Volcano plot showing differential protein abundance of proteins bound to astrocyte versus microglia immunopurified lipoprotein. Proteins involved in glucose metabolism (Pkm, HECT domain E3 ubiquitin protein ligase 4 [Hectd4], and ApoD) and acute phase response (fibronectin 1 [Fn1] and Serpina3n) are differentially abundant in astrocyte ApoE lipoproteins. Proteins involved in complement activation (C1qa, C1qb, and complement factor I [CfI]), synapse pruning (C1qa and C1qb), proteolysis (matrix metallopeptidase 12 [Mmp12], serine carboxypeptidase 2 [Cpb2], CfI, and cathepsin B [Ctsb]), and the innate immune response (C1qa, C1qb, and CfI) are differentially abundant in microglia ApoE lipoprotein. For proteomics analysis, the following groups were analyzed: ApoE2 astrocytes (n = 5), ApoE3 astrocytes (n = 5), ApoE4 astrocytes (n = 5), ApoE2 microglia (n = 3), ApoE3 microglia (n = 3), and ApoE4 microglia (n = 4). B: PCA of the proteome of astrocyte and microglia ApoE lipoproteins. Circles