Figure 3Figure 3
Longevity mutants have reduced ribosome biogenesis. ( a ) FIB-1::GFP is strongly down-regulated in eat-2(ad465) animals but up-regulated in ncl-1(e1942) and eat-2;ncl-1 double mutants (20 worms imaged per replicate, 3 independent biological replicates). ( b – d ) rRNA, RPS6, RPS15 and FIB-1 levels are increased in ncl-1(e1942) and reduced in eat-2(ad465) , isp-1(qm150) , glp-1(e2141) , daf-2 RNAi and TOR RNAi and this effect is partially reversed by the loss of ncl-1. daf-2(e1370) also shows reduced rRNA levels compared to daf-2;daf-16(mu86) (Error bars represent mean±s.e.m.). ( e ) fib-1 RNAi extends lifespan of N2 ( P =0.0004, log-rank test, three independent biological replicates) ( f ) fib-1 RNAi reduces the nucleolar size of N2 (Error bars represent mean±s.d.). Scale bar represents 20 μm. * P <0.05, ** P <0.01, **** P <0.0001, unpaired t -test.