Figure 2Figure 2
The cytosolic DNA sensor cGAS is activated after cerebral ischemia/reperfusion. ( A ) The mRNA level of cGAS in primary microglia isolated from brain tissue 3 h post-tMCAO (9 mice per group). ( B ) The protein level of cGAS in primary microglia isolated from brain tissue at 1, 2, 3 and 6 h post-tMCAO, respectively. The gray values were analyzed by using ImageJ and were normalized to Iba1. ( C ) BV2 cells were transfected with siRNA against cGAS and harvested 48 h post-transfection to quantify the mRNA level of cGAS. ( D ) cGAS-silenced and control BV2 cells were transfected with poly(dA:dT) and harvested 3 h post-poly(dA:dT) treatment to analyze the protein levels of pIRF3, cGAS, and GAPDH. ( E ) cGAS-silenced, STING-silenced, and control BV2 cells were transfected with poly(dA:dT) and harvested 6 h post-poly(dA:dT) treatment to analyze the mRNA level of IFN-β. ( F ) BV2 cells were transfected with siRNA against STING and harvested 48 h post-transfection to quantify the mRNA level of