Fig. 2Figure 2
Prostate tissue visualised using tissue biopsies. a , b . Histological sections made from benign prostatic hyperplasia (BPH, with normal glandular structure embedded in stroma). Prostate cancer development is clinically described as a series of Gleason grades (1–5, with 1 corresponding to well-differentiated tissue containing a glandular structure and 5 being the most advanced with only few glands still visible) (Gleason and Mellinger 1974 ; Mellinger et al. 1967 ). c , d Histological sections made from prostate cancer (Gleason grade 5, notice breakdown of glandular structure). Left panels, sections processed using H&E staining. Right panels, sections processed by staining with haematoxylin and counterstaining with the RNA-binding protein Sam68 ( brown stain ). Figure adapted from (Rajan et al. 2008 ) with permission from the Journal of Pathology