Fig. 5Figure 5
Microglial Tbk1 deletion is sufficient to induce an FTD-like social recognition deficit. a Workflow of the 3-chamber test to assess presence of sociability and social recognition (social novelty preference) defects in 4-month-old male mice with microglial specific deletion of one (Tbk1-µG-HET) or two (Tbk1-µG-KO) Tbk1 copies and compared to control littermates (Tbk1-µG-WT). Created in BioRender. Lenoel, I. (2025) https://BioRender.com/ct8qnd6 . b Interaction times during the first three trials (trials 1–3) of the 3-chamber test (sociability phase). n = 12 mice per genotype (males only). Adjusted p values are indicated and were determined by a two-tailed type II Wald chi-square test with Tukey’s post hoc test. Results from trials 1–3 indicate no impairment of social interaction (sociability) due to microglial Tbk1 deletion. c Representative heatmaps of the mouse positions during the social recognition phase of the 3-chamber test (trial 4), shown for Tbk1-µG-KO and contro