Figure 6Figure 6
Expression of the resolvin receptors in the spinal cord. A and B, ChemR23 expression in the ipsilateral dorsal horn of the lumbar spinal cord (L3–L5) on day 14 ( A ) and day 35 ( B ) in MIA‐treated rats compared with saline‐treated rats. C and D, ALX expression in the ipsilateral dorsal horn of the lumbar spinal cord (L3–L5) on day 14 ( C ) and day 35 ( D ) in MIA‐treated rats compared with saline‐treated rats. Bars show the mean ± SEM (n = 5–6 per group). ∗ = P < 0.05; ∗∗ = P < 0.01 by unpaired t ‐test. E, Anti–glial fibrillary acidic protein (GFAP) immunofluorescence in the ipsilateral L4 dorsal horn of the spinal cord 28 days following induction of the MIA model of OA pain, indicative of increased astrocyte reactivity. Repeated systemic treatment with 17(R)‐HDoHE (300 ng every other day from day 14 to day 28 after model induction) resulted in a significant decrease in MIA‐induced GFAP immunofluorescence. The boxed area shows the area evaluated for GFAP quantification.