Fig. 2Figure 2
Amcp inhibited the proliferation of human pancreatic cancer cells. a The chemical structure of Amcp. b The time- and dose-dependent inhibitory effects of Amcp on two human pancreatic cancer cell lines, BxPC-3 and SW1990, in vitro. Cells were treated with Amcp at concentrations of 0.625, 1.25, 2.5, 5, and 10 µM for either 24, 48, or 72 h before the CCK-8 assay. c DAPI staining of the cell nucleus. Cells were treated with the indicated compounds for 24 h before fixation and stained with DAPI stain. Fluorescence was observed by using microscopy (Nikon Eclipse Ti). The magnification is ×400. Scale bar = 20 µm.