Fig. 3Figure 3
Amcp induced apoptosis in pancreatic cancer cells. a Amcp induced apoptosis in BxPC-3 and SW1990 cells. Cells were treated with Amcp for 24 h before collection. Cells were then stained with Annexin V/PI and analyzed by flow cytometry. b Apoptotic cells were quantified by GraphPad Prism 5. c Cells were treated with Amcp or gemcitabine for 24 h before Western blotting assays. Three independent experiments were performed and the data were presented as the mean ± SD. * P < 0.05, ** P < 0.01.