Fig. 5Figure 5
Amcp disrupted the mitochondrial membrane potential and affected mitochondrial proteins. a BxPC-3 and SW1990 cells were seeded into six-well plates at a density of 2 × 10 5 /well and were cultured for 24 h. After treatment with Amcp for 12 h, JC-1 staining was used to observe the depolarized mitochondrial membrane potential. Cells were analyzed by flow cytometry. b Quantitative analysis of cells with a depolarized mitochondria membrane. c Cells were treated with the indicated concentrations of Amcp or gemcitabine (Gem) for 24 h before Western blotting assays. * P < 0.05. d The protein level of Noxa was dramatically reduced by Noxa siRNA knockdown in BxPC-3 cells. e BxPC-3 cells were exposed to Amcp for 24 h with or without Noxa knockdown. Cell viability was determined by CCK-8 assays. f Noxa was knocked down by siRNA in BxPC-3 cells before treatment and cells were exposed to the indicated concentrations of Amcp or gemcitabine for 24 h before Western blotting analysis. g B