Fig. 1Figure 1
Retinal I/R injury significantly increases C1q expression in the retina. C57BL/6 J mice were subjected to unilateral I/R injury and sacrificed at the indicated time points post I/R. Contralateral control eyes ( a ) displayed basal deposits of C1q in the GCL; however, ischemic eyes ( b – f ) had significantly greater expression of C1q as early as day 3 ( b ) and sustained to the end of the time course on day 28 ( f ). Positive labeling was only observed in the GCL and IPL. ( g ) Fluorescence intensity quantification revealed statistically significant upregulation of C1q ( p < 0.01) at all time points in ischemic versus uninjured retinas. Peak intensity in ischemic eyes was observed on day 21 post injury ( p < 0.05). Mean ± SEM, n = 7 per group. ** p < 0.01 determined by student’s paired t -test, # p < 0.05 tested using One-way ANOVA followed by Holm-Sidak post test. GCL = Ganglion Cell Layer, INL = Inner Nuclear Layer, ONL = Outer Nuclear Layer. Scale bars = 50 μm