Figure 2Figure 2
Knockdown of ARHGEF11 induces cell growth inhibition, cell cycle arrest and cell apoptosis in hepatoma cell lines. ( A ) ARHGEF11 knockdown with two different siRNAs (siARHGEF11-1, siARHGEF11-1) or negative control (siNC) as examined by western blot and qRT-PCR. ( B ) The viability of hepatoma cells detected by CCK8 assay. ( C ) Cell cycle distribution detected by flow cytometry. The representative images (left) and percentage of cell cycle (right) are shown. ( D ) Cell apoptosis detected by flow cytometry. The representative images (left) and relative percentage of apoptotic cells (right) are shown. Data are shown as mean ± SD. * P <0.05, ** P <0.01.