Extended Data Fig. 10Figure 15
AQP4-directed autoimmunity in mice recapitulates histological hallmarks of NMOSD. ( a ) Representative CD45, AQP4, and GFAP stainings of the spinal cord, cerebellum, and ependyma of P41-immunized Aqp4 ΔB mice at the peak of EAE (scale bars top row 1 mm except for spinal cord 200 µm, all other rows 100 µm). ( b ) AQP4 loss was determined in a semi-quantitative approach normalized to the extent of adjacent CNS lesions defined by CD45 + immunoreactivity. The AQP4 signal was calculated by QuPath’s positive pixel count algorithm in the adjacent area with a defined radius of 100 µm with respect to CD45 + infiltrates (region of interest). Data are shown as mean ± SD and tested with a two-tailed unpaired t-test. * P < 0.05, ** P < 0.01, *** P < 0.001. Symbols represent individual CNS lesions detected in two biological replicates for each group.
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