FIGURE 2Figure 2
Differentially expressed adhesion (IACs and ECM) proteins (DEPs) in DU145 RR compared to DU145 P cells. (A) Protein-protein interaction network of DEPs and (B) volcano plot of proteins that passed the threshold SC ≥ 4 in two out of three replicas in DU145 P and/or RR cells (419 proteins). Matrisome proteins, including CCDC80 and TFPI2, are highlighted. Dashed lines represent 0.05 FDR (statistical significance after QSpec analysis)-value (horizontal line) and 1.5 (green) and 2-fold (red) change cut-off values (vertical lines). (C) WB validation of selected adhesion proteins from DU145 P and DU145 RR cells. Forty-eight hours after seeding, integrin nexus proteins were isolated, and WB analysis was performed. Fold change for selected DEPs obtained by WB (densitometry) is calculated by using ImageJ and amido-black as a reference. Selected proteins whose expression does not change are also included in WB validation. The table shows the spectral counts for selected DEPs obtained by MS.