FIGURE 5Figure 5
Frataxin silencing causes SR Ca 2+ store depletion in cardiomyocytes. (A) Loading of Fura2-AM. (B,D,F) FxnKD cells show a marked difference to 1 μM thapsigargin response compared to Scr. (C–G) The amplitude of the response in relative values % shows a significant decrease in FxnKD cells which reveals a decrease of Ca 2+ content in the SR ( ∗ p < 0.05; ∗∗ p < 0.005). Scale bar (20 mm).