FIGURE 7Figure 7
FxnKD causes an abnormal energetic response to caffeine in cardiomyocytes. (A) TMRM was loaded in de-quence mode, at high concentration, where a depolarization of ΔΨ m is seen by an increase of fluorescence. The response to caffeine depolarises the potential as the mitochondria are active taking up Ca 2+ to restore the Ca 2+ homeostasis of the cell. (B,C) In FxnKD cells the response is reduced revealing that the mitochondria may not take up Ca 2+ correctly ( ∗ p < 0.05). Scale bar (20 μm).