Fig. 1Figure 2
Single-cell atlas of cardiac immune cells during sepsis progression. a , Schematic diagram showing scRNA-seq pipeline of murine cardiac immune cells during sepsis progression. SS, steady state. b , Uniform Manifold Approximation and Projection (UMAP) plots of 29,537 cardiac immune cells allocated into 15 clusters from the hearts of 14 WT mice at SS, 3, 7 and 21 d after CLP. Cardiac cells from 3–4 mice were mixed as one sample. c , UMAP plots of cardiac immune cells colored by cell type. d , Heat map showing selected cluster marker genes (top, color-coded by cluster and condition) with exemplar genes and cell-type annotation labeled. e , UMAP plots of cardiac immune cells from WT mice in different time points of sepsis, annotated by cell type as in c . Each time point is contributed by either three (SS or 21 d) or four mice (3 or 7 d). f , Bar plots showing the distribution of immune cell types from WT mice at different time points of sepsis (Extended Data Figs. 1 and 2 ). Sour