Extended Data Fig. 2Figure 3
Single-cell characterization and flow cytometric analysis of the cardiac immune cells during sepsis progression, related to Fig. 1 . a, Violin plots of average unique molecular identifier (UMI) (left) and gene (right) numbers of scRNA-seq at different stages of sepsis. Each dot represents a cell. b, UMAP plots showing the expression of selected marker genes for the defined cell types in Fig. 1c . c, Violin plots showing the expression of selected marker genes in all defined cell types corresponding to Fig. 1c . d, Gating strategy of 10-color flow cytometry for identifying various types of immune cells in murine hearts. e, Graph showing percentages of cardiac immune cells during sepsis progression (n = 4 - 5 mice for each treatment). Each symbol represents one animal in e. Data are presented as mean ± SEM. Two-sided P values were determined by one-way ANOVA followed by Tukey’s multiple comparisons test, n.s., not significant. In e, experiments were performed four times.
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