TREM2 Ectodomain Variant — Stability-Optimized

🧬 Protein Design

Method

Rosetta Relax

Stability

91%

Binding (Kd)

780 nM

pLDDT

0.89

ΔG

-2.3 kcal/mol

MUTATIONS (3)

T96SK186RA192V

Sequence (278 aa)

MEPLRPGWLLLVSLAQVALGGPPPGPRPGLWAWEGRVREGPRLPAAGTAVPPPGAVWQLEDGRTMCFARHGPLSLGADVAAVSWLRGAVGRGLAAAVLLAVALLVLWLTRRAGRSRKDTIEVPVEEPPHVFVVFTDADTHPKELLLFCTQSDLCFQAQQSVHQWAEFNGSSVVPDCCHPLHPGSSLVGALLREPQEVLLHRLPDAIQGLSLVIKDVQEGDMFHCVWQDWGMPSPQMPQQVEPAYHPKVFAGQVMVTQCVQHDCFALRQPSGREPDHPG

Rosetta-guided mutations to improve thermostability without disrupting binding interface

🔮 3D Structure — AlphaFold Q9NZC2 Click to load

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