{"artifact":{"id":"experiment-exp-cf95a475-75fb-403f-9b98-f0f3deeb45e5","artifact_type":"experiment","entity_ids":"[]","title":"Germfree mouse colonocyte energy metabolism analysis","quality_score":0.9,"created_by":"experiment_extractor","provenance_chain":"[]","content_hash":"6c9b766e10a14f7c47298ce9d6f547f689acda9c5dc652ef80f6983ed9e5ce95","metadata":{"_origin":{"url":null,"type":"internal","tracked_at":"2026-04-10T23:13:52.204066"},"p_value":null,"protocol":"Comparison of metabolic parameters between germfree and conventional mouse colonocytes, including enzyme expression analysis, NADH/NAD+ ratio measurement, ATP quantification, AMPK and p27(kip1) phosphorylation assessment, and autophagy marker detection","effect_size":null,"sample_size":null,"source_pmid":"21531334","extracted_at":"2026-04-10T16:13:52.199462","_schema_version":1,"experiment_type":"animal_model","success_criteria":"Demonstration of decreased metabolic enzyme expression, reduced energy production, and activation of energy-sensing pathways in germfree colonocytes","expected_outcomes":"Germfree colonocytes would show signs of energy deprivation due to lack of bacterial butyrate","statistical_methods":null},"created_at":"2026-04-10T23:13:52-07:00","updated_at":"2026-04-10T23:13:52-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"internal","origin_url":null,"lifecycle_changed_at":"2026-04-10T23:13:52-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":"ok","contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"f7a3c3f8-40cd-4dd8-80af-3f04e890bd51","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"experiment_extractor"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[{"target_artifact_id":"wiki-therapeutics-nad-precursors-neurodegeneration","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: NAD"},{"target_artifact_id":"wiki-cell-types-p2x3-receptor-neurons","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: ATP"},{"target_artifact_id":"wiki-proteins-ampk-protein","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: AMPK"},{"target_artifact_id":"wiki-mechanisms-nad-metabolism-neurodegeneration","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: NAD"},{"target_artifact_id":"wiki-therapeutics-nad-boosters-neurodegeneration","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: NAD"},{"target_artifact_id":"wiki-mechanisms-nad-signaling-neurodegeneration","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: NAD"},{"target_artifact_id":"wiki-mechanisms-ampk-activators-parkinsons","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: AMPK"},{"target_artifact_id":"wiki-mechanisms-autophagy","link_type":"related","strength":0.54,"evidence":"Reverse: Title match: autophagy"},{"target_artifact_id":"wiki-proteins-prkaa1-protein","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: AMPK"},{"target_artifact_id":"wiki-therapeutics-nmn-nad-precursor-parkinsons","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: NAD"},{"target_artifact_id":"wiki-entities-ampk","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: AMPK"},{"target_artifact_id":"wiki-mechanisms-nad-metabolism-pathway-neurodegeneration","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: NAD"},{"target_artifact_id":"wiki-therapeutics-ampk-activators-neurodegeneration","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: AMPK"},{"target_artifact_id":"wiki-therapeutics-nad-precursor-therapy","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: NAD"},{"target_artifact_id":"wiki-mechanisms-nad-bioenergetics-investment-synthesis","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: NAD"},{"target_artifact_id":"wiki-entities-autophagy","link_type":"related","strength":0.54,"evidence":"Reverse: Title match: Autophagy"},{"target_artifact_id":"wiki-proteins-nadk-protein","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: NAD"},{"target_artifact_id":"wiki-investment-ampk-activators","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: AMPK"},{"target_artifact_id":"wiki-therapeutics-nad-boosters","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: NAD"},{"target_artifact_id":"wiki-therapeutics-ampk-activator-therapies","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: AMPK"},{"target_artifact_id":"wiki-mechanisms-ampk-signaling-parkinsons","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: AMPK"},{"target_artifact_id":"wiki-experiments-nad-augmentation-therapy-early-ad","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: NAD"},{"target_artifact_id":"wiki-therapeutics-ampk-activators","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: AMPK"},{"target_artifact_id":"wiki-mechanisms-ampk-neurodegeneration","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: AMPK"},{"target_artifact_id":"wiki-ideas-payload-ampk-agonist-neurodegeneration","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: AMPK"},{"target_artifact_id":"wiki-mechanisms-ampk-signaling-pathway","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: AMPK"},{"target_artifact_id":"wiki-mechanisms-ampk-mitochondrial-quality-control","link_type":"related","strength":0.63,"evidence":"Reverse: Gene mention: AMPK"}],"incoming_links":[{"source_artifact_id":"wiki-therapeutics-nad-precursors-neurodegeneration","link_type":"mentions","strength":0.7,"evidence":"Gene mention: NAD"},{"source_artifact_id":"wiki-cell-types-p2x3-receptor-neurons","link_type":"mentions","strength":0.7,"evidence":"Gene mention: ATP"},{"source_artifact_id":"wiki-proteins-ampk-protein","link_type":"mentions","strength":0.7,"evidence":"Gene mention: AMPK"},{"source_artifact_id":"wiki-mechanisms-nad-metabolism-neurodegeneration","link_type":"mentions","strength":0.7,"evidence":"Gene mention: NAD"},{"source_artifact_id":"wiki-therapeutics-nad-boosters-neurodegeneration","link_type":"mentions","strength":0.7,"evidence":"Gene mention: NAD"},{"source_artifact_id":"wiki-mechanisms-nad-signaling-neurodegeneration","link_type":"mentions","strength":0.7,"evidence":"Gene mention: NAD"},{"source_artifact_id":"wiki-mechanisms-ampk-activators-parkinsons","link_type":"mentions","strength":0.7,"evidence":"Gene mention: AMPK"},{"source_artifact_id":"wiki-mechanisms-autophagy","link_type":"mentions","strength":0.6,"evidence":"Title match: autophagy"},{"source_artifact_id":"wiki-proteins-prkaa1-protein","link_type":"mentions","strength":0.7,"evidence":"Gene mention: AMPK"},{"source_artifact_id":"wiki-therapeutics-nmn-nad-precursor-parkinsons","link_type":"mentions","strength":0.7,"evidence":"Gene mention: NAD"},{"source_artifact_id":"wiki-entities-ampk","link_type":"mentions","strength":0.7,"evidence":"Gene mention: AMPK"},{"source_artifact_id":"wiki-mechanisms-nad-metabolism-pathway-neurodegeneration","link_type":"mentions","strength":0.7,"evidence":"Gene mention: NAD"},{"source_artifact_id":"wiki-therapeutics-ampk-activators-neurodegeneration","link_type":"mentions","strength":0.7,"evidence":"Gene mention: AMPK"},{"source_artifact_id":"wiki-therapeutics-nad-precursor-therapy","link_type":"mentions","strength":0.7,"evidence":"Gene mention: NAD"},{"source_artifact_id":"wiki-mechanisms-nad-bioenergetics-investment-synthesis","link_type":"mentions","strength":0.7,"evidence":"Gene mention: NAD"},{"source_artifact_id":"wiki-entities-autophagy","link_type":"mentions","strength":0.6,"evidence":"Title match: Autophagy"},{"source_artifact_id":"wiki-proteins-nadk-protein","link_type":"mentions","strength":0.7,"evidence":"Gene mention: NAD"},{"source_artifact_id":"wiki-investment-ampk-activators","link_type":"mentions","strength":0.7,"evidence":"Gene mention: AMPK"},{"source_artifact_id":"wiki-therapeutics-nad-boosters","link_type":"mentions","strength":0.7,"evidence":"Gene mention: NAD"},{"source_artifact_id":"wiki-therapeutics-ampk-activator-therapies","link_type":"mentions","strength":0.7,"evidence":"Gene mention: AMPK"},{"source_artifact_id":"wiki-mechanisms-ampk-signaling-parkinsons","link_type":"mentions","strength":0.7,"evidence":"Gene mention: AMPK"},{"source_artifact_id":"wiki-experiments-nad-augmentation-therapy-early-ad","link_type":"mentions","strength":0.7,"evidence":"Gene mention: NAD"},{"source_artifact_id":"wiki-therapeutics-ampk-activators","link_type":"mentions","strength":0.7,"evidence":"Gene mention: AMPK"},{"source_artifact_id":"wiki-mechanisms-ampk-neurodegeneration","link_type":"mentions","strength":0.7,"evidence":"Gene mention: AMPK"},{"source_artifact_id":"wiki-ideas-payload-ampk-agonist-neurodegeneration","link_type":"mentions","strength":0.7,"evidence":"Gene mention: AMPK"},{"source_artifact_id":"wiki-mechanisms-ampk-signaling-pathway","link_type":"mentions","strength":0.7,"evidence":"Gene mention: AMPK"},{"source_artifact_id":"wiki-mechanisms-ampk-mitochondrial-quality-control","link_type":"mentions","strength":0.7,"evidence":"Gene mention: AMPK"},{"source_artifact_id":"wiki-mechanisms-autophagy","link_type":"related","strength":0.55,"evidence":"Pathway match: TCA cycle, oxidative phosphorylation, AMPK signaling, autophagy"}],"current_artifact_id":"experiment-exp-cf95a475-75fb-403f-9b98-f0f3deeb45e5","is_canonical":true,"supersede_chain":["experiment-exp-cf95a475-75fb-403f-9b98-f0f3deeb45e5"]}