{"artifact":{"id":"paper-fig-31989025-2","artifact_type":"paper_figure","entity_ids":"[]","title":"Figure 2: Gch1 overexpression and its downstream metabolites BH4/BH2 rescue from ferroptos...","quality_score":0.5,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"31989025\", \"relation\": \"extracted_from\"}]","content_hash":"31f139f4dae0acd7d765ad155a30bec9fa962fefbb564953fa97714a0eb7c8be","metadata":{"doi":"","pmcid":"PMC6978838","_origin":{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978838/figure/fig2/","type":"external","tracked_at":"2026-04-21T19:28:25.610320"},"caption":"Gch1 overexpression and its downstream metabolites BH4/BH2 rescue from ferroptosis. (A) Survival of MF-dCas9-Gch1 (Gch1 OE) compared to empty vector MF control (control) cells and GCH1-IRES-Puro (GCH1 OE) cells compared to parental HT-1080 cells (parental), respectively, against inducers of cell death: Ferroptosis induced with 2 μM IKE and Gpx4–/– by using 1 μM tamoxifen with 10 μM α-tocopherol (αToc) rescue. Extrinsic apoptosis induced by 20 ng/mL tumor necrosis factor α (TNFα) with 10 μM z-VAD-FMK (zVAD) rescue. Intrinsic apoptosis induced by 20 μM doxorubicin (Doxo), 25 μM etoposide (Etop), and 12.5 μM colchicine (Colch). Necroptosis induced by 1 μg/mL lipopolysaccharide (LPS) cotreatment with 10 μM zVAD with 10 μM necrostatin-1 (Nec-1) rescue. (B) Dose response curves of BH2 and BH4 starting at 200 μM rescuing from ferroptosis in MF control and parental HT-1080 cells induced by 1 μM RSL3, 2 μM IKE, and Gpx4–/– by 1 μM tamoxifen. (C) Effect of BH2 and BH4 on lipid peroxidation induc","paper_id":"31989025","image_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978838/figure/fig2/","image_path":"","description":"","figure_label":"Figure 2","figure_number":2,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-21T12:28:25.610409-07:00","updated_at":"2026-04-21T12:28:25.610409-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978838/figure/fig2/","lifecycle_changed_at":"2026-04-21T12:28:25.610409-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"b1c409d6-025c-47f2-b289-643f64ada5c6","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-31989025-2","is_canonical":true,"supersede_chain":["paper-fig-31989025-2"]}