{"artifact":{"id":"paper-fig-36747024-2","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 2: SPP1 is expressed by PVMs and fibroblasts. a – c , Representative images of  Spp...","quality_score":0.95,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"36747024\", \"relation\": \"extracted_from\"}]","content_hash":"dead08a5b8ca2bd036d9623b212af334eb9aafe7331178f080351cdbb3ba1df9","metadata":{"doi":"","pmcid":"PMC9991912","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC9991912/bin/41593_2023_1257_Fig2_HTML.jpg","type":"external","tracked_at":"2026-04-15T23:28:06.212381"},"caption":"SPP1 is expressed by PVMs and fibroblasts. a – c , Representative images of  Spp1  mRNA expression juxtaposed to GLUT1 +  vasculature, colocalizing with pan-PVM markers  Cd163  ( a ), CD206 ( b ) and PVF ( Pdgfra + ) ( c ) in 6-month  App NL-F  SLM as characterized by smFISH-IHC. Scale bar represents 10 µm. Data are representative of four  App NL-F  mice examined over two independent experiments.  d – f , Representative FACS plots to identify PVMs (CX3CR1 + CD45 + CD11b + CD206 +  ( d ), microglia CX3CR1 high CD45 + CD11b +  ( e ) or gated on total TdT expressing cells isolated from  Spp1 TdT  hippocampal homogenates and quantification ( f )). One datapoint represents one cell type per mouse (PVM, PVF and microglia) pooled from  n  = 7 mice examined over two independent experiments ( f ).  P  values from one-way ANOVA, Bonferroni’s multiple comparison test.  g , Three-dimensional reconstruction of CD206 +  PVMs expressing SPP1-Td along GLUT1 +  vessels in SLM from  Spp1 TdT  mice. Scal","paper_id":"36747024","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC9991912/bin/41593_2023_1257_Fig2_HTML.jpg","image_path":"","description":"","figure_label":"Fig. 2","figure_number":2,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-15T23:28:06-07:00","updated_at":"2026-04-16T21:02:10.792794-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC9991912/bin/41593_2023_1257_Fig2_HTML.jpg","lifecycle_changed_at":"2026-04-15T23:28:06-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":"ok","contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"3e2a47d6-577c-4ba4-870b-2a9a91524ca9","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[{"target_artifact_id":"paper-36747024","link_type":"derives_from","strength":1.0,"evidence":"paper_figure metadata/provenance references PMID 36747024"}],"incoming_links":[],"current_artifact_id":"paper-fig-36747024-2","is_canonical":true,"supersede_chain":["paper-fig-36747024-2"]}