{"artifact":{"id":"paper-fig-36747024-3","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 3: Spp1  modulates complement activation and microglial synaptic engulfment upon ac...","quality_score":0.95,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"36747024\", \"relation\": \"extracted_from\"}]","content_hash":"984dcb284928146819a591a1967d50fc6acd5ecf7b00dd68c99bd3c7f097bf1a","metadata":{"doi":"","pmcid":"PMC9991912","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC9991912/bin/41593_2023_1257_Fig3_HTML.jpg","type":"external","tracked_at":"2026-04-15T23:28:06.227578"},"caption":"Spp1  modulates complement activation and microglial synaptic engulfment upon acute oAβ challenge. a , Scheme illustrating ICV injection of S26C oAβ versus PBS in WT versus  Spp1 KO/KO  mice, 18 h before tissue collection and analysis.  b , Quantification of SPP1 immunoreactivity within SLM of 3-month WT mice injected with oAβ versus PBS control, at either 18 h or 72 h post-ICV injection. One datapoint represents one ROI per mouse hippocampus, with total of  n  = 3 mice per genotype and time point, examined over one independent experiment.  P  values from two-way ANOVA, Bonferroni’s multiple comparison test.  c , Representative images of C1q expression in SLM of 3-month PBS versus oAβ-injected WT mice. Scale bar represents 20 µm.  d , Representative 3D reconstructed images showing Homer1 engulfment within CD68 +  lysosomes of P2Y12 +  microglia from WT mice injected with oAβ. Scale bar represents 5 µm.  e , Representative images of C1q expression in  Spp1 KO/KO  mice injected with PBS ","paper_id":"36747024","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC9991912/bin/41593_2023_1257_Fig3_HTML.jpg","image_path":"","description":"","figure_label":"Fig. 3","figure_number":3,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-15T23:28:06-07:00","updated_at":"2026-04-16T21:02:10.792794-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC9991912/bin/41593_2023_1257_Fig3_HTML.jpg","lifecycle_changed_at":"2026-04-15T23:28:06-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":"ok","contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"797112d9-6c15-4865-98b5-5aea33bb521b","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[{"target_artifact_id":"paper-36747024","link_type":"derives_from","strength":1.0,"evidence":"paper_figure metadata/provenance references PMID 36747024"}],"incoming_links":[],"current_artifact_id":"paper-fig-36747024-3","is_canonical":true,"supersede_chain":["paper-fig-36747024-3"]}