{"artifact":{"id":"paper-fig-36747024-4","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 4: SPP1 drives microglial engulfment of synapses in AD context. a – d , Representat...","quality_score":0.95,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"36747024\", \"relation\": \"extracted_from\"}]","content_hash":"350a59da9b7777c146da86fbd3bc698ffde15d4cfead71e7c46ab60ce581b2cf","metadata":{"doi":"","pmcid":"PMC9991912","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC9991912/bin/41593_2023_1257_Fig4_HTML.jpg","type":"external","tracked_at":"2026-04-15T23:28:06.243481"},"caption":"SPP1 drives microglial engulfment of synapses in AD context. a – d , Representative image of 3D reconstructed P2Y12 +  microglia expressing phagocytic markers  Ctsb  ( a , b ) and  Grn  ( c , d ) assessed by smFISH-IHC in 6-month WT,  Spp1 KO/KO , App NL-F  and  App NL-F · Spp1 KO/KO  SLM. Scale bar represents 7 µm. Quantification of  Ctsb  ( b ) and  Grn  ( d ) mRNA levels expression within P2Y12 +  microglia. One datapoint represents average of 1 mouse, calculated from 6 individual P2Y12 +  microglia per mouse ( b ) and 3–6 microglia ( d ) from  n  = 3 mice per genotype examined over 2 independent experiments.  P  Values from two-way ANOVA, Bonferroni’s multiple comparison test for ( b ).  e , Representative 3D reconstructed images showing Homer1 engulfment within CD68 +  lysosomes of P2Y12 +  microglia in 6-month  App NL-F  versus  App NL-F · Spp1 KO/KO  SLM. Scale bar represents 7 µm.  f , Quantification of Homer1 engulfment ratio in P2Y12 +  microglia of WT versus  Spp1 KO/KO  ver","paper_id":"36747024","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC9991912/bin/41593_2023_1257_Fig4_HTML.jpg","image_path":"","description":"","figure_label":"Fig. 4","figure_number":4,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-15T23:28:06-07:00","updated_at":"2026-04-16T21:02:10.792794-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC9991912/bin/41593_2023_1257_Fig4_HTML.jpg","lifecycle_changed_at":"2026-04-15T23:28:06-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":"ok","contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"995c8dcd-5d37-4f59-bdae-feb2ae318b35","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[{"target_artifact_id":"paper-36747024","link_type":"derives_from","strength":1.0,"evidence":"paper_figure metadata/provenance references PMID 36747024"}],"incoming_links":[],"current_artifact_id":"paper-fig-36747024-4","is_canonical":true,"supersede_chain":["paper-fig-36747024-4"]}