{"artifact":{"id":"paper-fig-36747024-9","artifact_type":"paper_figure","entity_ids":"[]","title":"Extended Data Fig. 4: Primary microglia engulf synaptosomes through extrinsic SPP1. (a)  Schematic ill...","quality_score":0.95,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"36747024\", \"relation\": \"extracted_from\"}]","content_hash":"4a84cfe6f1bf95c63adecb3657401d2a02bad2d2eecbb1b7719c0e04bb9f382d","metadata":{"doi":"","pmcid":"PMC9991912","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC9991912/bin/41593_2023_1257_Fig9_ESM.jpg","type":"external","tracked_at":"2026-04-15T23:28:06.320756"},"caption":"Primary microglia engulf synaptosomes through extrinsic SPP1. (a)  Schematic illustrating  in vitro  engulfment assay whereby primary microglia isolated from WT or  Spp1 KO/KO  mice phagocytose S26C oAβ-treated synaptosomes that are tagged with pHrodo upon PBS or recombinant SPP1 treatment.  (b)  Representative images showing engulfment of pHrodo-synaptosomes by primary microglia isolated from WT mice (upper panel) or  Spp1 KO/KO  mice (lower panel), treated with vehicle (left) or 100 nM recombinant SPP1 (right). Data are representative of primary microglial cultures prepared from n = 6−8 neonates per genotype, examined over 2 independent experiments. Scale bar represents 100 µm.  (c)  Representative FACS plot showing pHrodo signal in CD11b +  cells collected from primary microglial culture (upper). No pHrodo signal was observed after Bafilomycin treatment, confirming lysosomal acidification as the source of pHrodo signal (lower). Data are representative of 2 independent experiments.  ","paper_id":"36747024","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC9991912/bin/41593_2023_1257_Fig9_ESM.jpg","image_path":"","description":"","figure_label":"Extended Data Fig. 4","figure_number":9,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-15T23:28:06-07:00","updated_at":"2026-04-16T21:02:10.792794-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC9991912/bin/41593_2023_1257_Fig9_ESM.jpg","lifecycle_changed_at":"2026-04-15T23:28:06-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":"ok","contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"a8553971-74ea-472b-a069-34116ab84660","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[{"target_artifact_id":"paper-36747024","link_type":"derives_from","strength":1.0,"evidence":"paper_figure metadata/provenance references PMID 36747024"}],"incoming_links":[],"current_artifact_id":"paper-fig-36747024-9","is_canonical":true,"supersede_chain":["paper-fig-36747024-9"]}