{"artifact":{"id":"paper-fig-37245027-4","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 4: PA load in peripheral organs and brain regions.  a  Bacterial growth in lung and...","quality_score":0.95,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"37245027\", \"relation\": \"extracted_from\"}]","content_hash":"342c4daa5213a0f6fee83757cc370841729ff0f11b7f80d70ee301092c9e3469","metadata":{"doi":"","pmcid":"PMC10223932","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC10223932/bin/12974_2023_2817_Fig4_HTML.jpg","type":"external","tracked_at":"2026-04-16T11:31:39.686299"},"caption":"PA load in peripheral organs and brain regions.  a  Bacterial growth in lung and spleen (CFUs per mg of tissue) and brain (in CSF; CFU per μL) obtained from infected animals (OD 600  0.3) and controls after intratracheal administration of PA at 24 h, 7-days and 1-month post-infection.  b  Representative confocal micrograph showing ( b ) lung and ( c ) spleen sections obtained from GFP-labelled PA infected animals at 24 h post-infection (OD 600  0.3) and controls. PA is labelled in green and nuclei (DAPI) in blue.  d–g  Representative confocal micrographs of ( d ) cortical sections, ( e ) choroid plexus ( f ) hippocampus and ( g ) isolated capillaries showing the lack of distribution of PA (GFP-labelled PA) in the brain of infected mice at 24 h post-infection and controls. Lectin (red) was used to label the blood vessels in tissue sections and claudin-5 or ZO-1 (red) to label isolated capillaries. DAPI (blue) was used to stain the nuclei","paper_id":"37245027","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC10223932/bin/12974_2023_2817_Fig4_HTML.jpg","image_path":"","description":"","figure_label":"Fig. 4","figure_number":4,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-16T11:31:39-07:00","updated_at":"2026-04-16T21:02:10.792794-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC10223932/bin/12974_2023_2817_Fig4_HTML.jpg","lifecycle_changed_at":"2026-04-16T11:31:39-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":"ok","contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"98bd69f6-ebaf-4a08-bff0-34905ed2e89e","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[{"target_artifact_id":"paper-37245027","link_type":"derives_from","strength":1.0,"evidence":"paper_figure metadata/provenance references PMID 37245027"}],"incoming_links":[],"current_artifact_id":"paper-fig-37245027-4","is_canonical":true,"supersede_chain":["paper-fig-37245027-4"]}