{"artifact":{"id":"paper-fig-39427196-2","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 2: LPS and proinflammatory cytokines suppress brain endothelial Wnt/β-catenin signa...","quality_score":0.95,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"39427196\", \"relation\": \"extracted_from\"}]","content_hash":"52977286ecdd31a9e93d80041387e50815fbf8640cbee92bd5a59a9054e9d7a6","metadata":{"doi":"","pmcid":"PMC11491032","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC11491032/bin/12974_2024_3261_Fig2_HTML.jpg","type":"external","tracked_at":"2026-04-16T11:31:41.884779"},"caption":"LPS and proinflammatory cytokines suppress brain endothelial Wnt/β-catenin signaling.  A-B , Co-immunofluorescence staining of active β-catenin (non-phosphorylated) and CD31 in the brain cortex tissue of mice treated with LPS or saline ( A ) and its quantifications ( B ). Active β-catenin signal in endothelial cells was selectively quantified.  n  = 6 mice per group. ( C-D ) Co-immunofluorescence staining of LEF1 and CD31 in the brain cortex tissue of mice treated with LPS or saline ( C ) and its quantifications ( D ).  n  = 6 mice per group.  E , RT-qPCR analysis of the expression levels of Wnt/β-catenin signaling target genes  Axin2 ,  Apcdd1 ,  Nkd1 , and  Notum  in brain endothelial cells (ECs) isolated by flow cytometry from saline or LPS-treated mice (normalized to β-actin).  n  = 7 mice per group.  F-H , RT-qPCR analysis of the expression levels of  Axin2  in mouse brain microvascular ECs (bEnd.3) treated with recombinant Wnt3a protein (100 ng/ml) or Wnt3a protein plus LPS ( F )","paper_id":"39427196","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11491032/bin/12974_2024_3261_Fig2_HTML.jpg","image_path":"","description":"","figure_label":"Fig. 2","figure_number":2,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-16T11:31:41-07:00","updated_at":"2026-04-16T20:55:17.546747-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11491032/bin/12974_2024_3261_Fig2_HTML.jpg","lifecycle_changed_at":"2026-04-16T11:31:41-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":"ok","contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"a9b18a9f-8f60-44db-83c4-82c3149f9714","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[{"target_artifact_id":"paper-39427196","link_type":"derives_from","strength":1.0,"evidence":"paper_figure metadata/provenance references PMID 39427196"}],"incoming_links":[],"current_artifact_id":"paper-fig-39427196-2","is_canonical":true,"supersede_chain":["paper-fig-39427196-2"]}