{"artifact":{"id":"paper-fig-39427196-3","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 3: NF-κB p65 interacts with β-catenin and reduces β-catenin-dependent gene transcri...","quality_score":0.95,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"39427196\", \"relation\": \"extracted_from\"}]","content_hash":"cdd2f9992499b3c6dd37da1cbe6a5e629a5d688f38121d44ffd929bed0b11230","metadata":{"doi":"","pmcid":"PMC11491032","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC11491032/bin/12974_2024_3261_Fig3_HTML.jpg","type":"external","tracked_at":"2026-04-16T11:31:41.906167"},"caption":"NF-κB p65 interacts with β-catenin and reduces β-catenin-dependent gene transcription in brain endothelium.  A-C , Immunofluorescence staining of active β-catenin (non-phosphorylated) in bEnd.3 cells treated with recombinant Wnt3a protein (100 ng/ml) only or Wnt3a protein plus TNF-α (100 ng/ml) or IL-1β (10 ng/ml) for 24 h and its quantifications presented as total signal intensity ( B ) and nuclear signal intensity ( C ).  D-E , Immunofluorescence staining of NF-κB p65 in bEnd.3 cells treated with TNF-α or IL-1β and its quantifications presented as nuclear intensity.  F , RT-qPCR analysis of the expression levels of Wnt target gene  Axin2  in bEnd.3 cells when NF-κB p65 was knocked down and the cells were treated with Wnt3a with or without TNF-α or IL-1β.  G , TOP-Flash assay of bEnd.3 cells treated with NF-κB pathway inhibitor BAY 11-7085 (10 mM) and the cells were treated with Wnt3a with or without TNF-α or IL-1β.  H , Bimolecular fluorescence complementation (BiFC) assay in HEK 293","paper_id":"39427196","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11491032/bin/12974_2024_3261_Fig3_HTML.jpg","image_path":"","description":"","figure_label":"Fig. 3","figure_number":3,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-16T11:31:41-07:00","updated_at":"2026-04-16T20:55:17.546747-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11491032/bin/12974_2024_3261_Fig3_HTML.jpg","lifecycle_changed_at":"2026-04-16T11:31:41-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":"ok","contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"3ca8942c-ccdc-47f0-904c-e68ca68c6a01","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[{"target_artifact_id":"paper-39427196","link_type":"derives_from","strength":1.0,"evidence":"paper_figure metadata/provenance references PMID 39427196"}],"incoming_links":[],"current_artifact_id":"paper-fig-39427196-3","is_canonical":true,"supersede_chain":["paper-fig-39427196-3"]}