{"artifact":{"id":"paper-fig-39427196-5","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 5: Genetic activation of endothelial Wnt/β-catenin signaling in vivo mitigates LPS-...","quality_score":0.95,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"39427196\", \"relation\": \"extracted_from\"}]","content_hash":"cfe9e262cf167bfa512ff44dc0fd968955c58ab44ade705731edad9e4629ae80","metadata":{"doi":"","pmcid":"PMC11491032","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC11491032/bin/12974_2024_3261_Fig5_HTML.jpg","type":"external","tracked_at":"2026-04-16T11:31:41.947975"},"caption":"Genetic activation of endothelial Wnt/β-catenin signaling in vivo mitigates LPS-induced BBB leakage and neuroinflammation.  A , Schematic illustration of experimental design for the induction of β-catenin (encoded by  Ctnnb1 ) constitutive activation in mice with LPS-induced endotoxemia.  B , RT-qPCR analysis of the expression levels of Wnt/β-catenin signaling target genes  Axin2 ,  Apcdd1 ,  Nkd1 , and  Notum  in brain endothelial cells (ECs) isolated by flow cytometry from WT or β-cat mice (normalized to β-actin).  C , Mortality rate of WT and β-cat mice at 24 h following Saline or LPS treatment. WT + Saline ( n  = 10 mice), WT + LPS ( n  = 16 mice), β-cat + LPS ( n  = 8 mice).  D , Body weight change of the mice at 24 h after Saline or LPS injection. WT + Saline ( n  = 6 mice), WT + LPS ( n  = 7 mice), β-cat + LPS ( n  = 6 mice).  E , Co-immunofluorescence staining of LEF1 and CD31 in the brain cortex and its qualifications.  F-G , Representative brain images showing Evans Blue leak","paper_id":"39427196","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11491032/bin/12974_2024_3261_Fig5_HTML.jpg","image_path":"","description":"","figure_label":"Fig. 5","figure_number":5,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-16T11:31:41-07:00","updated_at":"2026-04-16T20:55:17.546747-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11491032/bin/12974_2024_3261_Fig5_HTML.jpg","lifecycle_changed_at":"2026-04-16T11:31:41-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":"ok","contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"50bbc832-1cd1-423e-a5c7-17ccc8094506","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[{"target_artifact_id":"paper-39427196","link_type":"derives_from","strength":1.0,"evidence":"paper_figure metadata/provenance references PMID 39427196"}],"incoming_links":[],"current_artifact_id":"paper-fig-39427196-5","is_canonical":true,"supersede_chain":["paper-fig-39427196-5"]}