{"artifact":{"id":"paper-fig-40102948-4","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 4: The inhibition of NETs formation reduced BBB permeability in SAE mice by allevia...","quality_score":0.95,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"40102948\", \"relation\": \"extracted_from\"}]","content_hash":"850f8ff1ffe9252f5c15b335e67cd0fcf4abf86d83d47dea950b426798ee8e1d","metadata":{"doi":"","pmcid":"PMC11917101","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC11917101/bin/12974_2025_3395_Fig4_HTML.jpg","type":"external","tracked_at":"2026-04-16T11:31:44.217701"},"caption":"The inhibition of NETs formation reduced BBB permeability in SAE mice by alleviating TJs disruption.  A  The BWC was measured. Quantification of the FS extravasation in the hippocampus ( B ) and the global brain tissue ( C ) (n = 6).  D  Representative images showing the extravasation of FS.  E  Expression levels of TJs (ZO-1, Occludin, Claudin-5) proteins in the hippocampus were detected by Western blot (n = 6).  F  Expression levels of TJ proteins were quantified and normalized to GAPDH levels (n = 6). Double labeling shows colocalization of Occludin ( G ) and ZO-1 ( I ) with ECs (CD31) in the CA1 region of the hippocampus (40x, n = 6). Quantification of the fluorescence intensity of Occludin ( H ) and ZO-1 ( J ) in the hippocampus. NETs: Neutrophil extracellular traps; CLP: Cecal Ligation and Puncture; SAE: Sepsis-associated encephalopathy; ECs: endothelial cells; BBB: Blood–brain barrier; TJs: Tight junctions; BWC: Brain water content; FS: Fluorescein sodium. (ns: no significance, ","paper_id":"40102948","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11917101/bin/12974_2025_3395_Fig4_HTML.jpg","image_path":"","description":"","figure_label":"Fig. 4","figure_number":4,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-16T11:31:44-07:00","updated_at":"2026-04-16T20:55:17.546747-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11917101/bin/12974_2025_3395_Fig4_HTML.jpg","lifecycle_changed_at":"2026-04-16T11:31:44-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":"ok","contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"e56324a1-6d9e-4a61-859a-8c5dac3333a8","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[{"target_artifact_id":"paper-40102948","link_type":"derives_from","strength":1.0,"evidence":"paper_figure metadata/provenance references PMID 40102948"}],"incoming_links":[],"current_artifact_id":"paper-fig-40102948-4","is_canonical":true,"supersede_chain":["paper-fig-40102948-4"]}