{"artifact":{"id":"paper-fig-41279937-2","artifact_type":"paper_figure","entity_ids":"[]","title":"Figure 2.: Aggregation propensity and seeding activity of alpha-synuclein. (A)  High aggreg...","quality_score":0.5,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"41279937\", \"relation\": \"extracted_from\"}]","content_hash":"372d403dcf8383fde2100c5fb95d4fca0dac42441a6a7f8f20c83761b35b4890","metadata":{"doi":"","pmcid":"PMC12632551","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC12632551/bin/nihpp-2025.10.07.680938v1-f0002.jpg","type":"external","tracked_at":"2026-04-28T07:22:23.531419"},"caption":"Aggregation propensity and seeding activity of alpha-synuclein. (A)  High aggregation propensity of alpha-synuclein in the synucleinopathy (Syn) appendix compared to substantia nigra (SN). Aggregation propensity for each proteoform was estimated using PASTA 2.0. Robust linear regression was used for statistical analysis adjusting for diagnosis (synucleinopathy/control) and age, with aggregation propensity as dependent variable and tissue as independent variable.  (B)  Aggregation kinetics as measured by the assay demonstrated significantly increased average ThT fluorescence (AU) in synucleinopathy appendixes (n=16) over time compared to controls (n=15). This suggests the higher seeding activity of misfolded alpha-synuclein in synucleinopathy appendixes versus controls with no detectable seeding activity.  (C)  The maximum fluorescence (Fmax) recorded at the plateau of aggregation was significantly higher in synucleinopathy appendix as compared to controls (p<0.05). The graph includes d","paper_id":"41279937","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC12632551/bin/nihpp-2025.10.07.680938v1-f0002.jpg","image_path":"","description":"","figure_label":"Figure 2.","figure_number":2,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-28T00:22:23.531502-07:00","updated_at":"2026-04-28T00:22:23.531502-07:00","version_number":5,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC12632551/bin/nihpp-2025.10.07.680938v1-f0002.jpg","lifecycle_changed_at":null,"citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"3d18b74f-09db-48e9-b797-a89c47f4c2bf","artifact_dir":"data/scidex-artifacts/3d/3d18b74f-09db-48e9-b797-a89c47f4c2bf","primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":"2026-04-28T23:09:20.950884-07:00","hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-41279937-2","is_canonical":true,"supersede_chain":["paper-fig-41279937-2"]}