{"artifact":{"id":"paper-fig-5a8e8d29-aa3b-400b-8ab4-e7f72ce959a6-2","artifact_type":"paper_figure","entity_ids":"[]","title":"Figure 2:: Inhibition of miR-3148 could promote myocardial mitophagy and reduce apoptosis i...","quality_score":0.5,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"5a8e8d29-aa3b-400b-8ab4-e7f72ce959a6\", \"relation\": \"extracted_from\"}]","content_hash":"b43b438b719552109bd3296a4d28400f98f3a725e3439edbb9d403d006916da6","metadata":{"doi":"10.25259/Cytojournal_209_2024","pmcid":"PMC11932976","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC11932976/bin/Cytojournal-22-19-g002.jpg","type":"external","tracked_at":"2026-04-23T01:08:22.299360"},"caption":"Inhibition of miR-3148 could promote myocardial mitophagy and reduce apoptosis in AMI mice. (a and b) WB analysis was performed to examine the expression levels of PINK1, Parkin, Beclin1, p62, LC3 I and LC3 II proteins associated with mitophagy. (c) The PINK1 and Parkin expression were measured by qRT-PCR. (d and e) Levels of apoptosis-related proteins, including Caspase 9, Caspase 3, and Cyt C, were evaluated through WB analysis. (f) TUNEL staining of mice myocardium. Apoptotic cells that were TUNEL-positive appear brown, with hematoxylin staining revealing blue nuclei. Cells that were TUNEL-positive typically cannot be further stained with hematoxylin. Bar = 50 μm. (g) Comparison of the number of TUNEL positive cells of mice among groups. All analyses,  n  = 3 in each group;  ✶ :  P  < 0.05. WB: Western blot, AMI: Acute myocardial infarction, PINK1: PTEN-induced kinase 1, LC3: Microtubule-associated proteins 1A/1B light chain 3B, qRT-PCR: Quantitative reverse transcription polymerase","paper_id":"5a8e8d29-aa3b-400b-8ab4-e7f72ce959a6","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11932976/bin/Cytojournal-22-19-g002.jpg","image_path":"","description":"","figure_label":"Figure 2:","figure_number":2,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-22T18:08:22.299459-07:00","updated_at":"2026-04-22T18:08:22.299459-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11932976/bin/Cytojournal-22-19-g002.jpg","lifecycle_changed_at":"2026-04-22T18:08:22.299459-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"c2fff0fd-5f62-4520-a4a8-dac315f46338","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-5a8e8d29-aa3b-400b-8ab4-e7f72ce959a6-2","is_canonical":true,"supersede_chain":["paper-fig-5a8e8d29-aa3b-400b-8ab4-e7f72ce959a6-2"]}