{"artifact":{"id":"paper-fig-632e2e73-56b1-4816-8c3f-2c9f1dc97082-7","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 7: Functional validation of predicted phenotypic plasticity upon chemotherapy. A  A...","quality_score":0.5,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"632e2e73-56b1-4816-8c3f-2c9f1dc97082\", \"relation\": \"extracted_from\"}]","content_hash":"6c30ca52b9036f1fcfcda54956fc11cb48cb5d0ef069cc595df677315bac191d","metadata":{"doi":"10.1038/s41698-025-00890-0","pmcid":"PMC12000621","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC12000621/bin/41698_2025_890_Fig7_HTML.jpg","type":"external","tracked_at":"2026-04-23T01:08:18.179003"},"caption":"Functional validation of predicted phenotypic plasticity upon chemotherapy. A  Analysis of PAX8 mRNA expression, influence and relative protein expression in U87MG, U87MG-R50, and U87MG-OFF. Top panel: Representative Western blot (WB) detection of PAX8 protein and its predicted activity based on the regulatory network (colored dots under the WB), along with quantitative analysis ( n  > 3) of its relative protein level. Unprocessed scans of the WB are provided in Supplementary Fig.  3 . Bottom panel: mRNA expression and influence barplots of PAX8 and detection of the PAX8 protein by immunofluorescence in the same samples analyzed by WB.  B  mRNA expression levels of NKX2.5 in primary and recurrent gliomas, as analyzed using the Chinese Glioma Genome Atlas (CGGA,  http://www.cgga.org.cn/index.jsp ) cohort. NKX2.5 expression is significantly higher in grade IV gliomas compared to grade III and grade II gliomas.  C  Survival analysis of recurrent GBM patients (grade IV) based on NKX2.5 exp","paper_id":"632e2e73-56b1-4816-8c3f-2c9f1dc97082","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC12000621/bin/41698_2025_890_Fig7_HTML.jpg","image_path":"","description":"","figure_label":"Fig. 7","figure_number":7,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-22T18:08:18.179094-07:00","updated_at":"2026-04-22T18:08:18.179094-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC12000621/bin/41698_2025_890_Fig7_HTML.jpg","lifecycle_changed_at":"2026-04-22T18:08:18.179094-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"9d9a52e3-a9c8-4cdf-a011-39e2991cb1aa","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-632e2e73-56b1-4816-8c3f-2c9f1dc97082-7","is_canonical":true,"supersede_chain":["paper-fig-632e2e73-56b1-4816-8c3f-2c9f1dc97082-7"]}