{"artifact":{"id":"paper-fig-640f626a-ad3e-4ccf-a806-fbe18cc83b81-3","artifact_type":"paper_figure","entity_ids":"[]","title":"Figure 3.: Mechanisms of activity-regulated midbrain cholinergic neuron-to-glioma signaling...","quality_score":0.5,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"640f626a-ad3e-4ccf-a806-fbe18cc83b81\", \"relation\": \"extracted_from\"}]","content_hash":"514c351e43856bd1f1a30f7b579211d97b554235ab8ee7122580e4a16e35fef7","metadata":{"doi":"10.1016/j.cell.2025.05.031","pmcid":"PMC12396346","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC12396346/bin/nihms-2096866-f0004.jpg","type":"external","tracked_at":"2026-04-23T01:07:49.609204"},"caption":"Mechanisms of activity-regulated midbrain cholinergic neuron-to-glioma signaling (A) Quantification of glioma cell proliferation (EdU + /HNA + ) in DMG cells exposed to conditioned medium (CM) from LDT or PPN midbrain explants following cholinergic neuronal optogenetic stimulation. One-way analysis of variance (ANOVA) with Tukey’s post hoc analysis; ** p  < 0.01, *** p  < 0.001. Data = mean ± SEM;  n  = three independent experiments, each with three wells per condition; each data point represents the mean of three wells per condition for a given experiment. (B and C) Measurement of BDNF and acetylcholine levels in midbrain explant CM from (B) LDT and (C) PPN. Data = mean ± SEM;  n  = three independent experiments, each with three wells per condition; each data point represents the mean of three wells per condition for a given experiment. (D and E) Schematic of the experimental paradigm and representative image showing the recording site for GRAB-ACh3.0 sensor experiment, performed to m","paper_id":"640f626a-ad3e-4ccf-a806-fbe18cc83b81","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC12396346/bin/nihms-2096866-f0004.jpg","image_path":"","description":"","figure_label":"Figure 3.","figure_number":3,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-22T18:07:49.609319-07:00","updated_at":"2026-04-22T18:07:49.609319-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC12396346/bin/nihms-2096866-f0004.jpg","lifecycle_changed_at":"2026-04-22T18:07:49.609319-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"5efa8467-d880-4cac-8bd2-860182a5bb53","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-640f626a-ad3e-4ccf-a806-fbe18cc83b81-3","is_canonical":true,"supersede_chain":["paper-fig-640f626a-ad3e-4ccf-a806-fbe18cc83b81-3"]}