{"artifact":{"id":"paper-fig-640f626a-ad3e-4ccf-a806-fbe18cc83b81-4","artifact_type":"paper_figure","entity_ids":"[]","title":"Figure 4.: Direct effects of acetylcholine on DMG (A) Timeline for the generation of iPSC-d...","quality_score":0.5,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"640f626a-ad3e-4ccf-a806-fbe18cc83b81\", \"relation\": \"extracted_from\"}]","content_hash":"488c9a066b4dadffafe431edb8b8438000a4bdd4d20ec73f165109ffbaf9141e","metadata":{"doi":"10.1016/j.cell.2025.05.031","pmcid":"PMC12396346","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC12396346/bin/nihms-2096866-f0005.jpg","type":"external","tracked_at":"2026-04-23T01:07:49.643647"},"caption":"Direct effects of acetylcholine on DMG (A) Timeline for the generation of iPSC-derived cholinergic with viral transduction (AAV-VAChTe1-ChRmine::eYFP or AAV-VAChTe1::eYFP) for co-culturing with glioma cells. (B) Confocal micrographs showing cholinergic motor neurons generated hiPSCs of a healthy 12-year-old male and co-cultured with DMG cells of an 8-year old male. NF-H/M, turquoise; H3K27M, white; scale bars, 50 μm. (C) Quantification of glioma cell proliferation (EdU + /H3K27M + ) when co-cultured with hiPSC-derived cholinergic motor neurons paired with  in vitro  optogenetic stimulation. One-way analysis of variance (ANOVA) with Tukey’s post hoc analysis; **** p  < 0.0001, ** p  < 0.01, ns: non-significant. Data = mean ± SEM;  n  = four independent experiments, each with three wells per condition; each data point represents the mean of three wells per condition for a given experiment. (D) Three-dimensional rendering illustrating presynaptic cholinergic neuron (NF-H/M: white) with pr","paper_id":"640f626a-ad3e-4ccf-a806-fbe18cc83b81","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC12396346/bin/nihms-2096866-f0005.jpg","image_path":"","description":"","figure_label":"Figure 4.","figure_number":4,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-22T18:07:49.643741-07:00","updated_at":"2026-04-22T18:07:49.643741-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC12396346/bin/nihms-2096866-f0005.jpg","lifecycle_changed_at":"2026-04-22T18:07:49.643741-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"6e904660-27c4-45ad-81d3-508169f2d8bf","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-640f626a-ad3e-4ccf-a806-fbe18cc83b81-4","is_canonical":true,"supersede_chain":["paper-fig-640f626a-ad3e-4ccf-a806-fbe18cc83b81-4"]}