{"artifact":{"id":"paper-fig-707e4dfb-3896-4244-8e2a-7a749d45b168-4","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 4: ZFP64 directly bound to the promoters of GCH1 and FTH1 to promote their transcri...","quality_score":0.5,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"707e4dfb-3896-4244-8e2a-7a749d45b168\", \"relation\": \"extracted_from\"}]","content_hash":"510e197d123eddf9c5fee08ff1a1ad0aac2cf38a76e6278b5a306e72f40d3a85","metadata":{"doi":"10.1186/s12967-025-06246-3","pmcid":"PMC11871786","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC11871786/bin/12967_2025_6246_Fig4_HTML.jpg","type":"external","tracked_at":"2026-04-23T01:08:29.148225"},"caption":"ZFP64 directly bound to the promoters of GCH1 and FTH1 to promote their transcription. ( A ) Expression levels of ferroptosis-related molecules in TNBC cells were detected by RT-qPCR after transfection with shNC or shZFP64 for 48 h. ( B ) FTH1 level in DOX-resistant/sensitive TNBC tissues was evaluated by RT-qPCR. ( C ) Pearson correlation test detected the correlation between ZFP64 and FTH1 levels in DOX-resistant TNBC tissues. ( D ) RT-qPCR analysis of GCH1 level in DOX-resistant/sensitive TNBC tissues. ( E ) Pearson correlation test evaluated the correlation between ZFP64 and GCH1 levels in DOX-resistant TNBC tissues. ( F & G ) GCH1 and FTH1 expression was measured by RT-qPCR and Western blotting in DOX-treated TNBC cells co-cultured with or without CAF/R together with or without transfection with shNC or shZFP64. ( H - J ) JASPAR database predicted the potential binding sites of ZFP64 to GCH1 or FTH1 promoter. ( K ) The direct binding of ZFP64 to GCH1 or FTH1 promoter in TNBC cells","paper_id":"707e4dfb-3896-4244-8e2a-7a749d45b168","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11871786/bin/12967_2025_6246_Fig4_HTML.jpg","image_path":"","description":"","figure_label":"Fig. 4","figure_number":4,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-22T18:08:29.148352-07:00","updated_at":"2026-04-22T18:08:29.148352-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11871786/bin/12967_2025_6246_Fig4_HTML.jpg","lifecycle_changed_at":"2026-04-22T18:08:29.148352-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"1563ebcf-39d8-40af-b016-080821ac0269","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-707e4dfb-3896-4244-8e2a-7a749d45b168-4","is_canonical":true,"supersede_chain":["paper-fig-707e4dfb-3896-4244-8e2a-7a749d45b168-4"]}