{"artifact":{"id":"paper-fig-707e4dfb-3896-4244-8e2a-7a749d45b168-5","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 5: FTH1 or GCH1 overexpression abolished shZFP64-mediated promotion in ferroptosis ...","quality_score":0.5,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"707e4dfb-3896-4244-8e2a-7a749d45b168\", \"relation\": \"extracted_from\"}]","content_hash":"68eb7659a4e2fdeb2ae2dc635042d5b7ffe7ebfa0b9add0df5c4610d651da469","metadata":{"doi":"10.1186/s12967-025-06246-3","pmcid":"PMC11871786","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC11871786/bin/12967_2025_6246_Fig5_HTML.jpg","type":"external","tracked_at":"2026-04-23T01:08:29.187547"},"caption":"FTH1 or GCH1 overexpression abolished shZFP64-mediated promotion in ferroptosis and DOX sensitivity in TNBC cells. ( A - D ) TNBC cells were transfected with vector, FTH1 or GCH1 overexpressing plasmid for 48 h. FTH1 and GCH1 expression in TNBC cells was assessed by RT-qPCR and Western blotting. ( E ) CCK-8 assay detected the viability of TNBC cells transfected with shZFP64 together with FTH1 or GCH1 overexpressing plasmid in the presence of DOX. ( F ) Cell death was determined by PI staining via flow cytometry in TNBC cells transfected with shNC or shZFP64 together with FTH1 or GCH1 overexpressing plasmid in the presence of DOX. ( G - I ) MDA, Fe 2+ , and GSH levels were measured in TNBC cells transfected with shNC or shZFP64 together with FTH1 or GCH1 overexpressing plasmid in the presence of DOX. ( J ) Lipid ROS production was assessed by C11-BODIPY 581/591 probe via flow cytometry in TNBC cells transfected with shNC or shZFP64 together with FTH1 or GCH1 overexpressing plasmid in th","paper_id":"707e4dfb-3896-4244-8e2a-7a749d45b168","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11871786/bin/12967_2025_6246_Fig5_HTML.jpg","image_path":"","description":"","figure_label":"Fig. 5","figure_number":5,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-22T18:08:29.187634-07:00","updated_at":"2026-04-22T18:08:29.187634-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11871786/bin/12967_2025_6246_Fig5_HTML.jpg","lifecycle_changed_at":"2026-04-22T18:08:29.187634-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"5a9fb597-6673-4be3-80cc-3302aec8869c","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-707e4dfb-3896-4244-8e2a-7a749d45b168-5","is_canonical":true,"supersede_chain":["paper-fig-707e4dfb-3896-4244-8e2a-7a749d45b168-5"]}