{"artifact":{"id":"paper-fig-9a10f9ee-2201-47b2-a4ca-b169666a53d9-4","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 3: PDE4D5 expression is upregulated in human failing hearts and PDE4D5 induces hype...","quality_score":0.5,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"9a10f9ee-2201-47b2-a4ca-b169666a53d9\", \"relation\": \"extracted_from\"}]","content_hash":"26c53fe84d7e65d09671120aabd18d87c8dbab1da9142a2656d31b23b550f528","metadata":{"doi":"10.1016/j.redox.2025.103563","pmcid":"PMC11909752","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC11909752/bin/gr3.jpg","type":"external","tracked_at":"2026-04-23T01:08:31.300161"},"caption":"PDE4D5 expression is upregulated in human failing hearts and PDE4D5 induces hypertrophy and oxidative stress and inhibits mitophagy in NRVMs . A,  Representative immunoblots and quantification of PDE4D and PDE4D variants (PDE4D5, PDE4D3, and PDE4D9) expression in left ventricle tissues from healthy donors (n = 3) and heart failure (HF) patients (n = 7). NRVMs were infected with mCherry-PDE4D5 adenovirus (Ad-PDE4D5) or control adenovirus (Ad-Ctrl) for 48 h  (B  through  D ).  B,  The protein expressions of PDE4D and hypertrophy marker gene, ANP in NRVMs; n = 6 independent experiments.  C,  Immunofluorescence staining of α-actinin (green) and DAPI (blue) in NRVMs and quantification of myocytes area; n = 24 cells from 3 independent experiments. Scale bar, 20 μm.  D,  DCFH-DA staining and quantification of ROS levels in NRVMs; n = 5 independent experiments. Scale bar, 200 μm.  E,  MitoSOX staining and quantification of mitochondrial ROS levels in NRVMs infected with GFP-N-terminal-PDE4D5 a","paper_id":"9a10f9ee-2201-47b2-a4ca-b169666a53d9","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11909752/bin/gr3.jpg","image_path":"","description":"","figure_label":"Fig. 3","figure_number":4,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-22T18:08:31.300269-07:00","updated_at":"2026-04-22T18:08:31.300269-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11909752/bin/gr3.jpg","lifecycle_changed_at":"2026-04-22T18:08:31.300269-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"b4fa77b2-2771-47e2-859f-343dd753fd75","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-9a10f9ee-2201-47b2-a4ca-b169666a53d9-4","is_canonical":true,"supersede_chain":["paper-fig-9a10f9ee-2201-47b2-a4ca-b169666a53d9-4"]}