{"artifact":{"id":"paper-fig-9a10f9ee-2201-47b2-a4ca-b169666a53d9-6","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 5: SIRT1 knockdown abolishes the protection of PDE4D silencing on ISO-induced hyper...","quality_score":0.5,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"9a10f9ee-2201-47b2-a4ca-b169666a53d9\", \"relation\": \"extracted_from\"}]","content_hash":"f249ec7d2b8aa4befabd9edf22390fd1fe2469c8cfdc654867a5924de909c2bf","metadata":{"doi":"10.1016/j.redox.2025.103563","pmcid":"PMC11909752","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC11909752/bin/gr5.jpg","type":"external","tracked_at":"2026-04-23T01:08:31.372313"},"caption":"SIRT1 knockdown abolishes the protection of PDE4D silencing on ISO-induced hypertrophy and mitochondrial dysfunction in cardiomyocytes. A  through  F,  NRVMs were transfected with PDE4D siRNA (siPDE4D) and SIRT1 shRNA or negative control siRNA (siNC) for 48 h followed by vehicle or ISO (10 μM) treatment for another 24 h.  A,  Immunofluorescence staining of α-actinin (red) and DAPI (blue) and quantification of myocytes area in NRVMs; n = 24 cells from 3 independent experiments. Scale bar, 20 μm.  B,  FACS analyses of ΔΨm in NRVMs. A loss of ΔΨm was demonstrated by the ratio of JC-1 aggregates to JC-1 monomers; n = 3 independent experiments.  C,  DCFH-DA staining and quantification of cellular ROS levels in NRVMs; n = 8 independent experiments. Scale bar, 50 μm.  D,  MitoSOX staining and quantification of mitochondrial ROS levels in NRVMs; n = 8 independent experiments. Scale bar, 10 μm.  E , Representative immunofluorescence images of SIRT1 staining (red) and DAPI staining (blue) in NRV","paper_id":"9a10f9ee-2201-47b2-a4ca-b169666a53d9","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11909752/bin/gr5.jpg","image_path":"","description":"","figure_label":"Fig. 5","figure_number":6,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-22T18:08:31.372406-07:00","updated_at":"2026-04-22T18:08:31.372406-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11909752/bin/gr5.jpg","lifecycle_changed_at":"2026-04-22T18:08:31.372406-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"b2c9f87a-e2d0-49a2-94c8-54ca0b2defed","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-9a10f9ee-2201-47b2-a4ca-b169666a53d9-6","is_canonical":true,"supersede_chain":["paper-fig-9a10f9ee-2201-47b2-a4ca-b169666a53d9-6"]}