{"artifact":{"id":"paper-fig-b5f2bdf6-f2a3-4b70-a1ff-797e2ea667ca-5","artifact_type":"paper_figure","entity_ids":"[]","title":"Figure 5: Activation of HCAR2 mitigates pyroptosis and tight junction damage induced by S....","quality_score":0.5,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"b5f2bdf6-f2a3-4b70-a1ff-797e2ea667ca\", \"relation\": \"extracted_from\"}]","content_hash":"f785608d8a375580dabbd7d706de443c9610cde28f246dede360c7e79d4c708b","metadata":{"doi":"10.1002/advs.202411947","pmcid":"PMC11884543","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC11884543/bin/ADVS-12-2411947-g007.jpg","type":"external","tracked_at":"2026-04-23T01:13:47.058455"},"caption":"Activation of HCAR2 mitigates pyroptosis and tight junction damage induced by S. aureus in mMECs. a) Schematic representation of the  S. aureus  infection model in vitro. b) Immunofluorescence images of ZO‐1 and Occludin in mMECs, with scale bar representing 100 µm. c) TUNEL staining of mMECs, with red fluorescence indicating TUNEL‐positive cells, scale bar represents 200 µm. d) Quantitative analysis of TUNEL‐positive cells. e) LDH release assay to measure LDH levels in mMECs. f) Transmission electron microscopy (TEM) images showing cellular morphology of mMECs, with red arrows indicating mitochondria and blue arrows indicating cell membranes. The scale bar represents 5 µm. g) Western blot analysis of GSDMD expression in mMECs. h) Western blot analysis of NLRP3, ASC, and caspase‐1 expression in mMECs. i) Immunofluorescence staining for CK18 in primary mammary epithelial cells, with scale bar representing 100 µm. j) Western blot analysis of HCAR2 expression in primary mMECs. k) Western ","paper_id":"b5f2bdf6-f2a3-4b70-a1ff-797e2ea667ca","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11884543/bin/ADVS-12-2411947-g007.jpg","image_path":"","description":"","figure_label":"Figure 5","figure_number":5,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-22T18:13:47.058545-07:00","updated_at":"2026-04-22T18:13:47.058545-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11884543/bin/ADVS-12-2411947-g007.jpg","lifecycle_changed_at":"2026-04-22T18:13:47.058545-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"b41d09ba-497d-4088-9d58-feb66d85a9bf","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-b5f2bdf6-f2a3-4b70-a1ff-797e2ea667ca-5","is_canonical":true,"supersede_chain":["paper-fig-b5f2bdf6-f2a3-4b70-a1ff-797e2ea667ca-5"]}