{"artifact":{"id":"paper-fig-d46a46a8-3f6d-499f-aca4-f80e82536942-2","artifact_type":"paper_figure","entity_ids":"[]","title":"FIGURE 2: BNIP3L and BNIP3 are critical to the enhanced mitophagy in ovarian CSCs. (A) Dif...","quality_score":0.5,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"d46a46a8-3f6d-499f-aca4-f80e82536942\", \"relation\": \"extracted_from\"}]","content_hash":"7fcd651f2ae11c055bc0b46f53e74afc803564a35abab0b2d633c5e99730eb4f","metadata":{"doi":"10.1111/jcmm.70704","pmcid":"PMC12516242","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC12516242/bin/JCMM-29-e70704-g003.jpg","type":"external","tracked_at":"2026-04-22T23:25:26.854518"},"caption":"BNIP3L and BNIP3 are critical to the enhanced mitophagy in ovarian CSCs. (A) Differential expression of BNIP3 and BNIP3L in spheroid and adherent cultured OVCAR3 cells identified in dataset  GSE28799 . (B) BNIP3L and BNIP3 expression were assessed in a panel of adherent and spheroid cultured ovarian cancer cell lines. (C–E) Effect of BNIP3L or BNIP3 knockdown on mitophagy in spheroid cultured ovarian cancer cells. Spheroid cultured OVCAR3 and OVCAR4 cells were transfected with BNIP3L (C) or BNIP3 (D) shRNA individually or simultaneously (E). Whole cell lysates were analysed for BNIP3L and BNIP3 expression, with Vinculin, GAPDH, or Tubulin serving as a loading control. Mitochondria were isolated after cells were treated with Baf A1 for 18 h and LC3 levels were examined, with COX IV used as the mitochondrial loading control.","paper_id":"d46a46a8-3f6d-499f-aca4-f80e82536942","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC12516242/bin/JCMM-29-e70704-g003.jpg","image_path":"","description":"","figure_label":"FIGURE 2","figure_number":2,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-22T16:25:26.854644-07:00","updated_at":"2026-04-22T16:25:26.854644-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC12516242/bin/JCMM-29-e70704-g003.jpg","lifecycle_changed_at":"2026-04-22T16:25:26.854644-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"d93bc0d1-e98a-4bd1-b29c-841613a78c70","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-d46a46a8-3f6d-499f-aca4-f80e82536942-2","is_canonical":true,"supersede_chain":["paper-fig-d46a46a8-3f6d-499f-aca4-f80e82536942-2"]}