{"artifact":{"id":"paper-fig-f224acb2-095b-423e-980a-e4cb8c8f61c8-3","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 3: Deficiency of GAS6/AXL signal exacerbated BBB breakdown during JEV infection. WT...","quality_score":0.5,"created_by":"paper_figures_pipeline","provenance_chain":"[{\"source\": \"paper\", \"paper_id\": \"f224acb2-095b-423e-980a-e4cb8c8f61c8\", \"relation\": \"extracted_from\"}]","content_hash":"d845ada74aa3b65184f6cc618c0000aafc48310a9efa5001b76135aee0efea6e","metadata":{"doi":"10.1186/s12974-024-03225-1","pmcid":"PMC11411859","_origin":{"url":"https://www.ebi.ac.uk/europepmc/articles/PMC11411859/bin/12974_2024_3225_Fig3_HTML.jpg","type":"external","tracked_at":"2026-04-23T01:14:25.011557"},"caption":"Deficiency of GAS6/AXL signal exacerbated BBB breakdown during JEV infection. WT and AXL −/−  mice were infected with JEV 10 6  PFU via foot pad.  A . Viral copies in each brain sample were tested by qPCR. Data were normalized to β-actin and the relative change was calculated based on the viral level of WT-d3-1. At 3 dpi, WT  n  = 3, AXL −/− n  = 3; at 5 dpi, WT  n  = 4, AXL −/− n  = 3. Data are shown as mean ± SD.  B . The JEV E protein in the brain was detected by WB. At 3 dpi, WT  n  = 3, AXL −/− n  = 3; at 5 dpi, WT  n  = 4, AXL −/− n  = 3.  C . Qualitative detection of BBB permeability via EB staining at 3 and 5 dpi.  D . Quantitative assay of BBB permeability using NaF extravasation. NaF leakage was calculated based on the fluorescence ratio in the brain to blood. PBS: WT  n  = 5, AXL −/− n  = 3; 3 dpi: WT  n  = 12, AXL −/− n  = 12; 5 dpi: WT  n  = 4, AXL −/− n  = 3. The data represent the mean ± SD  E . Representative images show the co-localisation of endothelial cells (CD31, r","paper_id":"f224acb2-095b-423e-980a-e4cb8c8f61c8","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11411859/bin/12974_2024_3225_Fig3_HTML.jpg","image_path":"","description":"","figure_label":"Fig. 3","figure_number":3,"_schema_version":1,"source_strategy":"pmc_api","entities_mentioned":""},"created_at":"2026-04-22T18:14:25.011662-07:00","updated_at":"2026-04-22T18:14:25.011662-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":"https://www.ebi.ac.uk/europepmc/articles/PMC11411859/bin/12974_2024_3225_Fig3_HTML.jpg","lifecycle_changed_at":"2026-04-22T18:14:25.011662-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"bda9bae0-32f2-4eb8-81ea-c1c08cdccb96","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_pipeline"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-f224acb2-095b-423e-980a-e4cb8c8f61c8-3","is_canonical":true,"supersede_chain":["paper-fig-f224acb2-095b-423e-980a-e4cb8c8f61c8-3"]}