{"artifact":{"id":"paper-fig-paper-07c5d7883c4f-5","artifact_type":"paper_figure","entity_ids":"[]","title":"FIGURE 5 — Connexin43 Deficiency Leads to Ventricular Arrhythmias by Reprogramming Proline ","quality_score":0.5,"created_by":"paper_figures_tool","provenance_chain":"[{\"step\": \"paper_figures_backfill\", \"pmid\": \"paper-07c5d7883c4f\", \"timestamp\": \"2026-04-22T01:29:42.303710\"}]","content_hash":null,"metadata":{"pmid":"paper-07c5d7883c4f","caption":"ROS‐mediated abnormal calcium handling confers the arrhythmic phenotype of Cx43‐KO iPSC‐CMs. (A) Representative calcium (Ca 2+ ) transient waveforms recorded by ratiometric Fura‐2 imaging from WT and Cx43‐KO iPSC‐CMs. (B–H) Bar graphs to compare key parameters of calcium transients between WT and KO iPSC‐CMs, including diastolic calcium, calcium amplitude, peak calcium, maximal rising rate, maximal decay rate, time to peak, and transient duration 90.  n  = 40 cells. (I) Mitochondrial calcium analysis through Rhod‐2 staining in WT and Cx43‐KO iPSC‐CMs with or without ISO (100 n m , 2 h) stimulation, and with or without treatment of either Mito‐TEMPO (MT, 5 µ m , 72 h) or  N ‐acetyl‐ l ‐cysteine (NAC, 5 m m , 4 h).  n  = 4–6 independently biological repeats. (J) Representative action potential waveforms recorded by single‐cell patch clamp from Cx43‐KO iPSC‐CMs treated with phosphate‐buffered saline (PBS, vehicle), NAC (5 m m , 4 h) or proline (Pro, 100 n m , 24 h). Dash lines indicate 0 ","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC13045320/bin/ADVS-13-e16090-g004.jpg","paper_title":"Connexin43 Deficiency Leads to Ventricular Arrhythmias by Reprogramming Proline Metabolism.","figure_label":"FIGURE 5","figure_number":5,"_schema_version":1,"source_strategy":"pmc_api"},"created_at":"2026-04-21T18:29:40.747630-07:00","updated_at":"2026-04-21T18:29:40.747630-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":null,"lifecycle_changed_at":"2026-04-21T18:29:40.747630-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"24b0f03a-200a-43ba-b0f8-2078395bc6c0","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_tool"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-paper-07c5d7883c4f-5","is_canonical":true,"supersede_chain":["paper-fig-paper-07c5d7883c4f-5"]}