{"artifact":{"id":"paper-fig-paper-133bd96a1c04-5","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 5 — Prolonged anesthesia induces neuroinflammation and complement-mediated microglia","quality_score":0.5,"created_by":"paper_figures_tool","provenance_chain":"[{\"step\": \"paper_figures_backfill\", \"pmid\": \"paper-133bd96a1c04\", \"timestamp\": \"2026-04-22T01:29:41.590088\"}]","content_hash":null,"metadata":{"pmid":"paper-133bd96a1c04","caption":"Prolonged anesthesia enhanced microglia phagocytosis and chemotaxis and then triggered microglial synaptic engulfment resulting in synaptic loss.  A  Prolonged anesthesia decreased the expression of the mRNA of synaptic markers, including SYP, SYN1, and PSD95 ( n  = 3 per group).  B  Prolonged anesthesia increased the expression of the mRNA of complement markers, including C1qa and C3 ( n  = 3 per group).  C  Prolonged anesthesia increased the expression of the mRNA of the microglial phagocytosis marker (CD68) ( n  = 3 per group).  D  Prolonged anesthesia increased the expression of the microglial phagocytosis marker (CD68) at the protein level ( n  = 3 per group).  E  Immunoblotting band of CD68, C1qa, PSD95, SYN1, SYP, and β-actin ( n  = 3 per group).  F  Prolonged anesthesia increased the expression of C1qa.  G–I  Prolonged anesthesia decreased the expression of PSD95 ( G ), SYN1 ( H ), and SYP ( I ).  J  Prolonged anesthesia enhanced microglial phagocytosis. The iba1(green) positiv","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC9814183/bin/12916_2022_2705_Fig5_HTML.jpg","paper_title":"Prolonged anesthesia induces neuroinflammation and complement-mediated microglial synaptic elimination involved in neurocognitive dysfunction and anxiety-like behaviors.","figure_label":"Fig. 5","figure_number":5,"_schema_version":1,"source_strategy":"pmc_api"},"created_at":"2026-04-21T18:29:40.747630-07:00","updated_at":"2026-04-21T18:29:40.747630-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":null,"lifecycle_changed_at":"2026-04-21T18:29:40.747630-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"d616cbdd-fbc9-416c-a13b-d188aaa77268","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_tool"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-paper-133bd96a1c04-5","is_canonical":true,"supersede_chain":["paper-fig-paper-133bd96a1c04-5"]}