{"artifact":{"id":"paper-fig-paper-133bd96a1c04-7","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 7 — Prolonged anesthesia induces neuroinflammation and complement-mediated microglia","quality_score":0.5,"created_by":"paper_figures_tool","provenance_chain":"[{\"step\": \"paper_figures_backfill\", \"pmid\": \"paper-133bd96a1c04\", \"timestamp\": \"2026-04-22T01:29:41.288653\"}]","content_hash":null,"metadata":{"pmid":"paper-133bd96a1c04","caption":"Microglial depletion rescued cognitive dysfunction and ameliorated anxiety-like behaviors in SIN rats.  A  The schedule of the second experiment. We used chow with PLX3397 to feed rats for 21 days, depleting the microglial population.  B  iba1 (green) positive microglia were sharply depleted in the hippocampus after PLX3397 treatment. Scale bar = 200 μm.  C  PLX3397 treatment effectively eliminated microglial marker-iba1 expression in the hippocampus.  D  Statistical chart of iba1 expression between both groups ( n  = 4 per group).  E, F  Microglial depletion rescued cognitive dysfunction of SIN rats ( n  = 7 per group). In MWM, microglial depletion increased the number and time of entering the platform quadrant of SIN rats.  G, H  Microglial depletion ameliorated anxiety-like behaviors of SIN rats ( n  = 7 per group). In the OPF test, microglial depletion increased the time and number of entering the central zone of SIN rats.  I, J  Microglial depletion ameliorated anxiety-like behavi","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC9814183/bin/12916_2022_2705_Fig7_HTML.jpg","paper_title":"Prolonged anesthesia induces neuroinflammation and complement-mediated microglial synaptic elimination involved in neurocognitive dysfunction and anxiety-like behaviors.","figure_label":"Fig. 7","figure_number":7,"_schema_version":1,"source_strategy":"pmc_api"},"created_at":"2026-04-21T18:29:40.747630-07:00","updated_at":"2026-04-21T18:29:40.747630-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":null,"lifecycle_changed_at":"2026-04-21T18:29:40.747630-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"0216d205-0355-49e7-9667-9d7de548863c","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_tool"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-paper-133bd96a1c04-7","is_canonical":true,"supersede_chain":["paper-fig-paper-133bd96a1c04-7"]}