{"artifact":{"id":"paper-fig-paper-40258472-6","artifact_type":"paper_figure","entity_ids":"[]","title":"Fig. 5 — Resibufogenin protects against atherosclerosis in ApoE(-/-) mice through blockin","quality_score":0.5,"created_by":"paper_figures_tool","provenance_chain":"[{\"step\": \"paper_figures_backfill\", \"pmid\": \"paper-40258472\", \"timestamp\": \"2026-04-22T01:29:41.869699\"}]","content_hash":null,"metadata":{"pmid":"paper-40258472","caption":"RBG Inhibits Inflammation and NLRP3 Inflammasome Assembly Induced by LPS + ATP in BMDMs. (A and B) Concentrations of IL18 and IL1β in BMDM culture supernatants (n = 8). (C–H) Relative mRNA expression levels of NLRP3, NEK7, ASC, Caspase-1, IL18, and IL1β were measured in BMDMs (n = 4). (I-Q) Western blot analysis and statistical analysis of NLRP3, ASC total protein, ASC oligomerization, NEK7, Caspase-1, Cleaved Caspase-1, Pro IL18, and IL18 (n = 3). (R, U, V, W, X) Representative immunofluorescence images and statistical analysis of NLRP3, ASC, and Caspase-1 in BMDM (n = 3). (S and T) Representative images of NF-κB immunofluorescence in BMDMs. Ratio of NF-κB relative fluorescence intensity between the nucleus and cytoplasm (n = 3). * P  < 0.05,  ** P  < 0.01,  *** P  < 0.001,  **** P  < 0.0001; ns indicates not significant.","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC12869210/bin/gr5.jpg","paper_title":"Resibufogenin protects against atherosclerosis in ApoE(-/-) mice through blocking NLRP3 inflammasome assembly.","figure_label":"Fig. 5","figure_number":6,"_schema_version":1,"source_strategy":"pmc_api"},"created_at":"2026-04-21T18:29:40.747630-07:00","updated_at":"2026-04-21T18:29:40.747630-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":null,"lifecycle_changed_at":"2026-04-21T18:29:40.747630-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"b0afd00f-7465-481b-8f2a-c2a871f42228","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_tool"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[],"incoming_links":[],"current_artifact_id":"paper-fig-paper-40258472-6","is_canonical":true,"supersede_chain":["paper-fig-paper-40258472-6"]}