{"artifact":{"id":"paper-fig-paper-41665955-4","artifact_type":"paper_figure","entity_ids":"[]","title":"Figure 4 — Neutrophil-microglia interaction drives motor dysfunction in a neuromyelitis opt","quality_score":0.5,"created_by":"paper_figures_tool","provenance_chain":"[{\"step\": \"paper_figures_backfill\", \"pmid\": \"paper-41665955\", \"timestamp\": \"2026-04-22T01:29:42.309922\"}]","content_hash":null,"metadata":{"pmid":"paper-41665955","caption":"Neutrophil-derived C5a enhances NET production and activates microglia via C5aR1 signaling. ( A  and  B ) Gray matter of AQP4-IgG recipient mice, C5a immunoreactivity was not seen in GFAP +  astrocytes ( A ) and rarely seen in IBA1 +  microglia/macrophages ( B ). ( C ) Triple immunostaining of lumbar cord of AQP4-IgG recipient mice revealed C5a in neutrophils (MPO + , left) and monocytes (CCR2-GFP + , enhanced by anti-GFP-IgG, right). ( D ) The percentage of spinal cord cells expressing C5a in AQP4-IgG-infused mice ( n  = 4 mice per group). ( E ) Colocalization analysis of C5a and MPO signals in  C  using Zen software. ( F  and  G ) Confocal images of neutrophil (Ly6G, red) containing C5a (green in  F ), and C5 (green in  G ) after TNF-α incubation in vitro. Granule protein, MPO + , magenta; nuclear segmentation, DAPI + , blue. BF, bright-field. ( H ) Lumbar cords from IgG-infused mice were dissociated enzymatically and subjected to high parametric flow cytometric analysis. ( I ) t-SNE","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC13038209/bin/jci-136-199706-g004.jpg","paper_title":"Neutrophil-microglia interaction drives motor dysfunction in a neuromyelitis optica model induced by subarachnoid AQP4-IgG.","figure_label":"Figure 4","figure_number":4,"_schema_version":1,"source_strategy":"pmc_api"},"created_at":"2026-04-21T18:29:40.747630-07:00","updated_at":"2026-04-21T18:29:40.747630-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":null,"lifecycle_changed_at":"2026-04-21T18:29:40.747630-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"16dea4bb-49ba-4b21-b100-e930fec18a30","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_tool"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[{"target_artifact_id":"paper-41665955","link_type":"cites","strength":0.9,"evidence":"paper_figure.metadata.pmid=paper-41665955"}],"incoming_links":[],"current_artifact_id":"paper-fig-paper-41665955-4","is_canonical":true,"supersede_chain":["paper-fig-paper-41665955-4"]}