{"artifact":{"id":"paper-fig-paper-41665955-5","artifact_type":"paper_figure","entity_ids":"[]","title":"Figure 5 — Neutrophil-microglia interaction drives motor dysfunction in a neuromyelitis opt","quality_score":0.5,"created_by":"paper_figures_tool","provenance_chain":"[{\"step\": \"paper_figures_backfill\", \"pmid\": \"paper-41665955\", \"timestamp\": \"2026-04-22T01:29:42.310810\"}]","content_hash":null,"metadata":{"pmid":"paper-41665955","caption":"C5ar1  deficiency abrogates downstream microglial activation response to AQP4-IgG infusion. ( A ) Representative confocal images from lumbar cord of wild-type (WT) and  C5ar1 -/-  mice infused with normal IgG or AQP4-IgG. Lysosomal CD68 immunoreactivity (red) is more abundant in microglia (green) of WT recipients of AQP4-IgG than in  C5ar1 –/–  recipients. Imaris 3D rendering images illustrate the magnitude of lysosomal expansion. ( B ) ImageJ (NIH) analysis of the percentage area occupied by lysosome inside microglia in the lumbar cord of different experimental groups; treatment:  F (1, \n 20)  = 51.35,  P  < 0.0001; genotype:  F (1, \n 20)  = 14.17,  P  = 0.0066;  n  = 5–6 mice per group. ( C ) Sholl analysis of microglial branching revealed by Imaris AI-powered filament tracing, which counts the number of microglial filaments intersected by 1 μm spherical steps. Treatment:  F (77, \n 3,730)  = 39.35,  P  < 0.0001; radius:  F (3, \n 95)  = 17.02,  P  < 0.0001;  n  = 19–32 microglia from ","image_url":"https://www.ebi.ac.uk/europepmc/articles/PMC13038209/bin/jci-136-199706-g005.jpg","paper_title":"Neutrophil-microglia interaction drives motor dysfunction in a neuromyelitis optica model induced by subarachnoid AQP4-IgG.","figure_label":"Figure 5","figure_number":5,"_schema_version":1,"source_strategy":"pmc_api"},"created_at":"2026-04-21T18:29:40.747630-07:00","updated_at":"2026-04-21T18:29:40.747630-07:00","version_number":4,"parent_version_id":null,"version_tag":null,"changelog":null,"is_latest":1,"lifecycle_state":"active","superseded_by":null,"deprecated_at":null,"deprecated_reason":null,"dependencies":null,"market_price":0.5,"origin_type":"external","origin_url":null,"lifecycle_changed_at":"2026-04-21T18:29:40.747630-07:00","citation_count":0,"embed_count":0,"derivation_count":0,"support_count":0,"contradiction_count":0,"total_usage":0.0,"usage_score":0.5,"usage_computed_at":null,"quality_status":null,"contributors":[],"answers_question_ids":null,"deprecated_reason_detail":null,"deprecated_reason_code":null,"commit_sha":null,"commit_submodule":null,"last_mutated_at":"2026-05-16T14:51:34.657673-07:00","disputed_at":null,"gap_id":null,"mission_id":null,"intrinsic_priority":null,"effective_priority":null,"artifact_id":"b7bb539f-03d1-4a39-bb8a-1ab1ef0698bc","artifact_dir":null,"primary_filename":null,"accessory_filenames":null,"folder_layout_version":1,"migrated_to_folder_at":null,"hypothesis_id":null,"authorship":{"kind":"human","contributors":[{"role":"author","actor_ref":"paper_figures_tool"}]},"epistemic_tier":"T3_provisional","created_by_agent_id":null},"outgoing_links":[{"target_artifact_id":"paper-41665955","link_type":"cites","strength":0.9,"evidence":"paper_figure.metadata.pmid=paper-41665955"}],"incoming_links":[],"current_artifact_id":"paper-fig-paper-41665955-5","is_canonical":true,"supersede_chain":["paper-fig-paper-41665955-5"]}